5 edition of Methods for serum-free culture of cells of the endocrine system found in the catalog.
Includes bibliographies and index.
|Other titles||Serum-free culture of cells of the endocrine system.|
|Statement||editors, David W. Barnes, David A. Sirbasku, Gordon H. Sato.|
|Series||Cell culture methods for molecular and cell biology ;, v. 2|
|Contributions||Barnes, David W. 1949-, Sirbasku, David A. 1941-, Sato, Gordon.|
|LC Classifications||QH585 .M464 1984|
|The Physical Object|
|Pagination||xvi, 255 p. :|
|Number of Pages||255|
|LC Control Number||84007202|
In this study we report the successful generation of a liposarcoma-derived, novel, human adipocyte cell line, LS These cells have been in continuous culture since early , exhibit a gene expression pattern similar to that in primary human preadipocytes, and are capable of terminal differentiation. Using our culture method, we succeeded in generating mature pituitary endocrine cells such as corticotrophs, which secreted a substantial amount of ACTH in response to CRH.
How does the endocrine system communicate with cells? Chemical Messengers are released into the blood. Differences between rate of control in the nervous system versus the endocrine system? Nervous system is shorter; Endocrine lasts longer and takes longer to activate. What body processes do the Nervous System control. Cdiseases of the endocrine system are common, particularly in small animal practice. Cendocrine disorders are manifest by derangement of functions in target organs (eg alopecia with Cushing’s or hypothyroidism, seizures/coma caused by hyperinsulinism, bone fracture with hyperparathyroidism, etc).
It is important to distinguish between an endocrine gland, which discharges hormones into the bloodstream, and an exocrine gland, which secretes substances through a duct opening in a gland onto an external or internal body surface. Salivary glands and sweat glands are examples of exocrine glands. Both saliva, secreted by the salivary glands, and sweat, secreted by the sweat glands, act on. the ENDOCRINE SYSTEM Hormones are chemical messengers. They bind to specific target cells with receptors, regulate metabolism and the sleep cycle, and contribute to growth and development. The endocrine glands and organs secrete these hormones all over the body.
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Cell Culture Methods for Molecular and Cell Biology: Methods for Preparation of Media, Supplements, and Substrata for Serum-Free Animal Cell Culture, Volume 1; Methods for Serum-Free Culture of Cells of the Endocrine System, Volume 2; Methods for Serum-Free Culture of Epithelial and Fibroblastic Cells, Volume 3; Methods for Serum-Free Culture of Neuronal and Lymphoid Cells.
Additional Physical Format: Online version: Methods for serum-free culture of cells of the endocrine system. New York: A.R. Liss, © (OCoLC) Cell Culture Methods for Molecular and Cell Biology (4 Vols) Vol. 1 Methods for Preparation of Media, Supplements, and Substrata for Serum-Free Animal Cell Culture Vol.
2 Methods for Serum-Free Culture of Cells of the Endocrine System Vol. 3 Methods for Serum-Free Culture of Epithelial and Fibroblastic Cells Vol. 4 Methods for Serum-Free Culture of Neuronal and Lymphoid Size: KB.
Barnes, D., and Sato, G., a, Methods for growth of cultured cells in serum-free medium, m. – PubMed CrossRef Google Scholar. 1 - Assay Techniques Available for the Toxicologist. Radioimmunoassay (RIA) and other newer assay methodologies have added new weapons to the arsenal of endocrine toxicologists and have given the practitioners of toxicology tools with which to monitor the endocrine effects of toxic agents.
Availability of cell lines that can be propagated in serum-free, hormone-supplemented medium may aid in the study of the mechanisms by which hormones influence cell growth. View Show abstract. Book review Full text access How to grow your own cell: Cell culture methods for molecular and cell biology.
Vol I, Methods for preparation of media, supplements, and substrata for serum-free animal cell culture; Vol. 2, Methods for serum-free culture of cells of the endocrine system; Vol.
3, Methods for serum-free culture of epithelial and fibroblastic cells; Vol. 4, Methods for serum-free. Cell Culture Methods for Molecular and Cell Biology - Volume 1: Methods for Preparation of Media, Supplements, and Substrata for Serum-Free Animal Cell Culture, Editors, David W Barnes, David A.
Cells within pancreatic organoids express stem cell markers and those typical of the pancreatic exocrine system, including (A) Axin2, (B) Krt19, (C) Muc1, and (D) Pdx1. Relative quantiﬁcation (RQ) of each marker is reported relative to the 18S and TBP housekeeping ge nes and normalized.
Kidwell WR, Bano M, Salomon DS: Growth of normal mammary epithelium on collagen in serum-free medium. In: Barnes DM, Sirbasku DA, Sato GH (eds): Cell Culture Methods for Molecular and Cell Biology, Vol 2.
Methods for Serum-Free Culture of Cells of the Endocrine System. Alan R. Liss, Inc., New York,pp – Google Scholar. Free bio chemistry microbiology Books. Addiction; Allergy & Immunology Computer User's Guide, Molecular Biology Methods and Tissue Culture Methods.
Author(s): University of Maryland, Baltimore The Circulatory System, Lymphatic System And Immunity, The Digestive System, The Nervous System, Endocrine System, The Reproductive System, The.
Similarly, certain cells send chemical signals to other cells in the body that influence their behavior. This long-distance intercellular communication, coordination, and control is critical for homeostasis, and it is the function of the endocrine system.
An Overview of the Endocrine System. The endocrine system coordinates with the nervous system to control the functions of the other organ systems. Cells of the endocrine system produce molecular signals called hormones. These cells may compose endocrine glands, may be tissues or may be located in organs or tissues that have functions in addition to hormone production.
The endocrine system uses chemical signals called hormones to convey information from one part of the body to a distant part of the body. Hormones are released from the endocrine cell into the extracellular environment, but then travel in the bloodstream to target tissues.
This communication and response can take seconds to days. The nervous system receives and sends information through nerve cells (neurons) as electrical impulses. A nerve impulse can travel as fast as meters/second (m/sec), and it targets a specific part of the body, such as a cell. The other control system is the endocrine system.
Endocrine Methods contains descriptions of contemporary and cutting-edge methodologies in various areas of endocrinology, including receptor theory and immunologic techniques for endocrine research.
The book presents step-by-step procedures easily available to study the endocrine system and includes experts in their respective fields as. Imagawa, Y. Tomooka, J. Yang, R. Guzman, J. Richards, and S. Nandi. Isolation and serum-free cultivation of mammary epithelial cells within a collagen gel matrix.
In “Methods for Serum-Free Culture of Cells of the Endocrine System”. No headers. This chapter describes the endocrine system and its vital roles in communication, control, and homeostasis within the human body.
The focus is on the pituitary gland, as the master gland of the endocrine system, and three other endocrine glands: the thyroid gland, adrenal glands, and pancreas.
Principles of Animal Physiology Principles of Animal Physiology,Second Edition Second Edition continues to set a new standard for animal physiology books with its focus on animal diversity, its clear foundation in molecular and cell biology, its concrete examples throughout, and its fully integrated coverage of the endocrine system.
Harmful chemicals that disrupt the endocrine system and hormone regulation have been associated with obesity. Here the authors apply a human pluripotent stem cell-based platform to study the. The endocrine system is a network of glands and organs located throughout the body.
It’s similar to the nervous system in that it plays a vital role in controlling and regulating many of the.1,25(OH) 2 D 3 is the principal active hormonal form of vitamin D 3, and it is responsible for most of VD’s biological n D 3 is produced from 7-dehydrocholesterol, which is catabolized to 25(OH)D 3 and then 1,25(OH) 2 D mice, CYP2R1 is one of the major enzymes responsible for the hydroxylation of vitamin D 3 in liver tissue, and a reduction of more than 50% of the 25(OH.The endocrine system coordinates with the nervous system to control the functions of the other organ systems.
Cells of the endocrine system produce molecular signals called hormones. These cells may compose endocrine glands, may be tissues or may be located in organs or tissues that have functions in addition to hormone production.